Synthesis of Lewis X epitopes on plant N-glycans

G. Rouwendal, D. Florack, T. Hesselink, J. Cordewener, J. Helsper and D. Bosch

Carbohydr Res 2009;344(12):1487-93.

DOI PMID Cited by ~14

Glycoproteins from tobacco line xFxG1, in which expression of a hybrid beta-(1-->4)-galactosyltransferase (GalT) and a hybrid alpha-(1-->3)-fucosyltransferase IXa (FUT9a) is combined, contained an abundance of hybrid N-glycans with Lewis X (Le(X)) epitopes. A comparison with N-glycan profiles from plants expressing only the hybrid beta-(1-->4)-galactosyltransferase suggested that the fucosylation of the LacNAc residues in line xFxG1 protected galactosylated N-glycans from endogenous plant beta-galactosidase activity.